GSM4654882: SA11 induced 2 ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ASCL1
Cell type
Cell type Class
Neural
Cell type
SH-SY5Y
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma
Attributes by original data submitter
Sample
source_name
SA-ASCL1 inducible cells
treatment
Dox
cell type
SH-SY5Y
clone
S-A 2
biological replicate
2
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP and ChIP-seq were performed as described previously (22,23 from prev. version). Antibodies used were anti-ASCL1 (Abcam, cat# ab74065), anti-P300 (Santa-Cruz, cat# sc-585), and anti-IgG (Abcam, cat# ab6706). ChIP-seq experiments were performed in two separate clones of each of WT and S-A SH-SY5Y tetracycline-inducible stable cell lines induced with 1 μg/ml doxycycline for 24 hours, before cross-linking with 1% formaldehyde. ChIP-seq and the input libraries were prepared using the ThruPLEX® DNA-seq Kit (Rubicon Genomics, cat# R400406), following the manufacturers instructions.